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A’ Level Biology:-

The concentration of glucose in solutions can be measured using the glucose oxidase technique.

The solution to be tested is incubated with glucose oxidase and then with peroxidase and an indicator which changes colour when oxidised in the reaction. Peroxidase breaks down the hydrogen peroxide formed by the glucose oxidase, releasing oxygen which changes the colour of the indicator.

The intensity of the colour produced is directly proportional to the concentration of glucose in the solution.

Describe how you would use this method to compare the concentration of glucose in a test solution to a known standard concentration of glucose solution.