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In this assignment you will imagine yourself in the role of a recently hired Practice Manager for a physician office called Welby-Eichman healthCare (WeCare). The practice is located in a small urban area. Three of the physicians are Internal Medicine Physicians and the other two are Family Practice physicians. You previously worked at LiveWell Medical Center as the HIM Manager and were on the front line there as they adopted and deployed EPIC at LiveWell South Physician Clinic, and this gave you a hiring advantage at WeCare.

For this assignment, you will need to download and complete the worksheet below:

  • Worksheet: Module 09 Lab – Worksheet

 

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(2pts) Basic steps to clone a gene into a vector for expression: (1)PCRamplificationofthe gene, (2) digest (cut) both the double-stranded PCR product (insert) and plasmid vector with the same restriction enzyme(s) to create complementary “sticky ends”, (3) allow the digested insert and vector to anneal, and (4) ligate the annealed fragments together with DNA Ligase.

A. What additional step is required to generate a DNA template for PCR from a eukaryotic organism rather than a prokaryotic organism, and why?

B. Why is it necessary to re-circularize (ligate) a plasmid prior to transformation into cells?

 

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write my assignment 9316

Dear Tutor:

I need assistance with an expert in ‘strategic management’ to complete the following essay questions based on the agency’s strategic plan.  The response should be focused around the organization’s generic and diversification strategies, its international moves, and its ethics, social responsibility, and environmental sustainability practices. The topics should include the following:

  1. Your organization’s generic strategy or strategies. 
  2. Any diversification strategies the organization pursues.
  3. Any international moves made by your organization. Does it operate internationally? In what format(s)?
  4. What ethics policies and practices does your organization have in place? 
  5. Does your organization practice social responsibility? In what way(s)? 
  6. What are your organization’s environmental sustainability practices? 

If you can help prepare a 2000 word essay by 3/25/18.  If yes, then I will provide the subject agency.

 

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Many of you will have heard of Six Sigma management. What you may not realize is that the etymology of the term Six Sigma is rooted in statistics. As you should have seen by now, statisticians use the Greek letter sigma (σ) to denote a standard deviation. So when these Six Sigma people start talking about “six sigma processes,” what they mean is that they want to have processes where there are (at least) six standard deviations between the mean and what would be determined to be a failure. For example, you may be examining the output of a factory that makes airline grade aluminum. The average tensile strength of each piece is 65 ksi, and you view a particular output as a failure if the tensile strength is anything less than 64 ksi. If the standard deviation is less than .166, then the process is six sigma. The odds of a failure within a six sigma process are 3.4 in a million, which corresponds to the 99.9997% confidence level. When we are doing statistics, we usually use the 95% confidence level, which is roughly 2 sigmas.

In the case of the tensile strength of airline grade aluminum, 6 sigmas is probably a good level to be at—catastrophic failure on an airplane could open you up to lawsuits worth billions of dollars. But there are some other processes that you probably don’t need to be so certain about getting acceptable products from. Give some examples of random processes that are likely to be normally distributed, and say how many sigmas you think the process should be at.

 

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