I have already finished the first and second questions. Hope someone can help me solve the last one. Thank you so much！！

h] The regional government office that deals with passport applications is designing a processthat will check applications and issue the doctmtents. The number of applications to beprocessed is LEDU per week and the time available to process the applications is 4t] hours per week. i. What is the required cycle time for the process? {11] marks} " I i If the total work content of all the activities that make up the total task of checkingprocessing and issuing a passport is, on average, it] minutes; how many people will heneeded to meet demand? (Ifl‘r’fi’aTlETsT ‘ iii. The passport office has a ‘clear desk” policy that means that all desks must be clear ofwork by the end of the day. How man}.r applications should be loaded onto the processin the morning in order to ensure that every one is completed and desks are clear by the end of the day? {assume a working dag,r of 15 hours i or 45d minutes) 214.gll-maflfi‘j’fl’

Dr. Rich purchased two different commercially available primary polyclonal antibodies to a newlycharacterized microtubule motor protein kinesin Z. The immunogen used was full length kinesin Z purified frombovine brain. Both were generated in rabbit. He observed the following staining pattern followingimmunofluorescence staining of fibroblasts. Ignore the identical cell shapes, these are images from differentstained coverslips. Also, images are shown in negative contrast; White= black; black/grey = green emittedfluorescence from the fluorescein coupled goat anti-rabbit secondary antibody. Cell and nuclear perimeters areindicated by dotted lines; perimeters were determined by phase contrast imaging of the same cells. Phasecontrast imaging also verified that the large organelle positively stained with antibody 2 is the nucleus—whichwas also present (by phase) in the cells stained with antibody 1.Give two possible explanations in a phrase/sentence or two (there are several possibilities) for thedifference in immunostaining observed with the two different anti kinesin-Z antibodies.